
MCPrimers generates molecular cloning PCR primers

Use: mcprimers.pl [options] vector.txt sequence.fasta result.pr3

Options:  -help
          -stdout
          -filter
          -searchpaststart integer
          -searchbeforestop integer
          -clamp (both | 3prime)
          -maxchanges integer
          -excludedsites comma_seperated_list_with_no_blanks
          -primerfile primer3_file_name
          -vectorfile vector_file_name
          -seqfile FASTA_sequence.fasta
          -outfile result.pr3

Vector file is specified in Bio::Data::Plasmid::CloningVector
Sequence file must be DNA nucleotides in FASTA format
Results file has Primer3 output and extra data
'=' can be used in specifying parameter values on command line

Use at your risk. Check any solutions you obtain

